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Optimal nitrogen source for the recovery of phycoerythrin in discolored Rhodomonas sp.
https://fra.repo.nii.ac.jp/records/2008492
https://fra.repo.nii.ac.jp/records/20084921af2abe7-e4e0-4e0a-9f24-734097df3775
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
yamamoto et al Optimal nitrogen source for the recovery of phycoerythrin in discolored Rhodomonas sp..pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2024-06-24 | |||||
| タイトル | ||||||
| タイトル | Optimal nitrogen source for the recovery of phycoerythrin in discolored Rhodomonas sp. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
山本, 慧史
× 山本, 慧史× 徳田, 雅治 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Rhodomonas species are of interest as excellent aquaculture feed because of their ideal biochemical composition, especially their high protein content, resulting from the presence of intracellular phycoerythrin (PE). One of the most crucial issue with this species as a feed source is PE degradation under ambient nitrogen starvation, which leads to unpredictable growth defects and reduced nutritional value associated with cell discoloration. The aim of this study was to propose an optimal nitrogen source for the recovery of intracellular PE in discolored Rhodomonas sp. We supplied three different nitrogen sources, i.e., nitrate, ammonium, and urea, to PE-degraded cells and monitored changes in PE content and the expression of related genes. Supplementation with each nitrogen source increased cell density, with nitrate exhibiting the highest specific growth rate of 0.30 ± 0.02 day−1. The maximum PE content in each nitrogen group at 48 h after N supplementation was about 3.8 pg cell−1 with no major difference between groups. In contrast, the highest relative PE recovery rate of 5.73 ± 0.18-fold and the highest PE productivities of 0.32 ± 0.01 μg mL−1 h−1 were obtained in the nitrate group. Nitrogen transporter genes, including RhNrt2, RhAmt1, and RhDur3, were upregulated under nitrogen-starvation conditions, demonstrating the ability of this species to utilize each of the tested nitrogen sources. Furthermore, RhCpeb, which serves as a molecular indicator of PE biosynthesis, was significantly upregulated in all nitrogen-supplemented groups, with the strongest and fastest increase in expression in the nitrate group, emphasizing the potential of nitrate as the most favorable nitrogen source for PE recovery in this species. Our findings pave the way for informed strategies for the stable management and production of Rhodomonas species as high-protein feed in aquaculture. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Algal Research 巻 80, p. 103521 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
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| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 2211-9264 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.algal.2024.103521 | |||||
| 情報源 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | Local | |||||
| 関連識別子 | 23333020 | |||||
| 言語 | ja | |||||
| 関連名称 | 水産技術研究所 養殖部門 育種部 （南勢） | |||||
| 関連サイト | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/abs/pii/S2211926424001334 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | SMUR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_71e4c1898caa6e32 | |||||
