| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-07-19 |
| タイトル |
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タイトル |
Growth, Toxin Content and Production of Dinophysis Norvegica in Cultured Strains Isolated from Funka Bay (Japan) |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
diarrhetic shellfish poisoning (DSP); Dinophysis norvegica; Mesodinium rubrum; Teleaulax amphioxeia; pectenotoxin-2 (PTX2); dinophysistoxin-1 (DTX1); okadaic acid (OA) |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
長井, 敏
Basti, Leila
内田, 肇
栗林, 貴範
| en |
Kuribayahi, Takanori
Hokkaido Research Organization
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| ja |
栗林, 貴範
北海道立中央水産試験場
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| ja-Kana |
クリバヤシ, タカノリ
ホッカイドウリツチュウオウスイサンシケンジョウ
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Search repository
夏池, 真人
| en |
Natsuike, Masafumi
Hokkaido Research Organization
|
| ja |
夏池, 真人
北海道立函館水産試験場
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| ja-Kana |
ナツイケ, マサフミ
ホッカイドウリツハコダテスイサンシケンジョウ
|
Search repository
Sildever, Sirje
中山, 奈津子
Wai Mun Lum
| en |
Wai Mun Lum
Japan Fisheries Research and Education Agency
The University of Tokyo
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Search repository
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The successful cultivation of Dinophysis norvegica Claparède & Lachmann, 1859, isolated from Japanese coastal waters, is presented in this study, which also includes an examination of its toxin content and production for the first time. Maintaining the strains at a high abundance (>2000 cells per mL−1) for more than 20 months was achieved by feeding them with the ciliate Mesodinium rubrum Lohmann, 1908, along with the addition of the cryptophyte Teleaulax amphioxeia (W.Conrad) D.R.A.Hill, 1992. Toxin production was examined using seven established strains. At the end of the one-month incubation period, the total amounts of pectenotoxin-2 (PTX2) and dinophysistoxin-1 (DTX1) ranged between 132.0 and 375.0 ng per mL−1 (n = 7), and 0.7 and 3.6 ng per mL−1 (n = 3), respectively. Furthermore, only one strain was found to contain a trace level of okadaic acid (OA). Similarly, the cell quota of pectenotoxin-2 (PTX2) and dinophysistoxin-1 (DTX1) ranged from 60.6 to 152.4 pg per cell−1 (n = 7) and 0.5 to 1.2 pg per cell−1 (n = 3), respectively. The results of this study indicate that toxin production in this species is subject to variation depending on the strain. According to the growth experiment, D. norvegica exhibited a long lag phase, as suggested by the slow growth observed during the first 12 days. In the growth experiment, D. norvegica grew very slowly for the first 12 days, suggesting they had a long lag phase. However, after that, they grew exponentially, with a maximum growth rate of 0.56 divisions per day (during Days 24–27), reaching a maximum concentration of 3000 cells per mL−1 at the end of the incubation (Day 36). In the toxin production study, the concentration of DTX1 and PTX2 increased following their vegetative growth, but the toxin production still increased exponentially on Day 36 (1.3 ng per mL−1 and 154.7 ng per mL−1 of DTX1 and PTX2, respectively). The concentration of OA remained below detectable levels (≤0.010 ng per mL−1) during the 36-day incubation period, with the exception of Day 6. This study presents new information on the toxin production and content of D. norvegica, as well as insights into the maintenance and culturing of this species. |
|
言語 |
en |
| 書誌情報 |
en : Toxins
巻 15,
号 5,
p. 318,
発行日 2023-05-01
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| 出版者 |
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出版者 |
MDPI |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2072-6651 |
| PubMed番号 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
PMID |
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関連識別子 |
37235353 |
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言語 |
en |
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関連名称 |
Pub Med |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.3390/toxins15050318 |
| 情報源 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
Local |
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関連識別子 |
23342253 |
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言語 |
ja |
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関連名称 |
水産技術研究所 環境・応用部門 沿岸生態システム部 |
| 関連サイト |
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関連タイプ |
isSupplementTo |
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識別子タイプ |
URI |
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関連識別子 |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221361/ |
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言語 |
en |
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関連名称 |
PubMed Central |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
Nagai et al. Growth, Toxin content and production of Dinophysis norvegica.pdf (2.4 MB)
